To study the effects of anti-cancer, anti-metastasis and immune response improvement of herbal-acupuncture with Eclipta prostrata diffuse herba infusion solution(ELP-HAS), we injected ELP-HAS into Chok-samni(St36) of C57BL/6 mice implanted intravenously with B16-F10 melanoma.
We have reached the following conclusion through the effect on the number of CD25^+/CD4^+, CD8^+/CD3e^+, CD69^+/B220^+, NK1.1^+/CD3e^+ cells in mouse PBMCs, the effect on the pulmonary colony number, and the effect on MST(Median Survival Time) and ILS(Increase of Life Span) of C57BL/6 mice implanted intravenously with B16-F10 melanoma.
The results were obtained as follows:
1. In the experiment groups treated with ELP(Eclipta prostrata) Herbal-Acupuncture, the spleen cell proliferation in Balb/c mouse was significantly increased compared with control group.
2. In the experiment groups treated with ELP(Eclipta prostrata) Herbal-Acupuncture, the percentage of CD25^+/CD4^+, CD8^+/CD3e^+, CD69^+/B220^+, NK1.1^+/CD3e^+ cells in C57Bl/6 mouse PBMCs was increased compared with control group.
3. In the experiment groups treated with ELP(Eclipta prostrata) Herbal-Acupuncture, the pulmonary colony number of C57BL/ mice implanted intravenously with B16-F10 melanoma was decreased significantly compared with control group.
4. In the experiment groups treated with ELP(Eclipta prostrata)Herbal-Acupuncture, MST(Median Survival Time) and ILS(Increase of Life Span) of C57BL/6 mice implanted intravenously with B16-F10 melanoma were increased significantly compared with control group.
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